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KMID : 0829220090330060353
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Korean Journal of Oral and Maxillofacial Pathology
2009 Volume.33 No. 6 p.353 ~ p.358
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Effect of 5'-Nitro-Indirubinoxime on SGT Cell Adhesion and Migration to Type I Collagen Treatment
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Yoon Jung-Hoon
Ahn Sang-Gun
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Abstract
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We have previously shown that 5’-nitro-indirubinoxime (5’-NIO) has potent anti-tumor effect in various human cancer cells. But the potential anti-invasive effect of 5’-NIO in salivary gland cancer has not been studied yet. The goal of this study is to evaluate the effect of 5'-NIO on salivary gland adenocarcinoma SGT cell adhesion and migration to Type I collagen treatment. Western blot, adhesion and migration assay were performed to evaluate the impacts of 5’-NIO on the expression of MMP-2/-9 and its upstream signaling molecules after treatment of type ? collagen. SGT cell adhesion to type I collagen is significantly suppressed by 5’-NIO. 5’-NIO decreased expression of β1 integrin, phosphorylation of FAK, MMP-2/ -9 compared with type I collagen treatment. In addition, 5’-NIO inhibited the migration of SGT cells treated with type I collagen. These results suggest that 5’-NIO could effectively inhibit the invasion and migration of human SGT cells by downregulating the expression of β1 integrin and MMP-2/-9 and phosphorylation of FAK, Akt, and Erk. Adhesion and migration to type I collagen of SGT cells can be influenced through 5’-NIO..
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KEYWORD
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5’-nitro-indirubinoxime, Salivary gland cancer, Migration, Invasion, Type I collagen, β1 integrin signaling
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